2011
 |
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics Deniz Pekin, Yousr Skhiri, Jean-Christophe Baret, Delphine Le Corre, Linas Mazutis, Chaouki Ben Salem, Florian Millot, Abdeslam El Harrak, J. Brian Hutchison, Jonathan W. Larson, Darren R. Link, Pierre Laurent-Puig, Andrew D. Griffiths and Valérie Taly Lab Chip 11 (2011) , 2156-2166 |
New Generation of Amino Coumarin Methyl Sulfonate-Based Fluorogenic Substrates for Amidase Assays in Droplet-Based Microfluidic Applications
Woronoff G, El Harrak A, Mayot E, Schicke O, Miller OJ, Soumillion P, Griffiths AD, Ryckelynck M.
Anal Chem. Mar 17 2852–2857(2011)
2010
Quantitative cell-based reporter gene assays using droplet-based microfluidics
Jean-Christophe Baret, Yannick Beck, Isabelle Billas-Massobrio, Dino Moras and Andrew D.Griffiths
Chemistry and Biology (2010)In press
High-throughput screening of enzymes by retroviral display using droplet-based microfluidics
Granieri L., Baret J.C., Griffiths A.D. & Merten C.A.
Chem. Biol., 17(3), 229-235 (2010)
Link
Tiny droplets for high-throughput cell-based assays
Baret, J.-C. and Taly, V. In Unravelling more of cell genomics : let's use the micro and nanotools !, RCS publication edited by M.-C. Potier and N. Bontoux.
An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries
Clausell-Tormos J., Griffiths A.D. & Merten C.A.
Lab on a Chip (2010)
Link
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
Jeremy J. Agresti, Eugene Antipov, Adam R. Abate, Keunho Ahn, Amy C. Rowat, Jean-Christophe Baret, Manuel Marquez, Alexander M. Klibanov, Andrew D. Griffiths, and David A. Weitz
PNAS , 107 (9), 4004-4009 (2010)
Link
2009
Multistep microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme
Mazutis L., Baret J.C., Tracy P., Skhiri Y., Fallah-Araghi A., Ryckelynck M., and A.D. Griffiths
Lab on a chip, 9, 2902-2908 (2009)
Link
Preparation of monodisperse emulsions by hydrodynamic size fractionation
Mazutis L. and Griffiths A.D.
Appl. Phys. Lett. 95, 204103 (2009)
Link
A remote syringe for cells, beads and particle injection in microfluidic systems
Baret J.-C.,
Lab on a chip, Chips and Tips (2009)
Link
A fast and efficient microfluidic system for highly selective one to one droplet fusion
Mazutis L., Baret J.-C., and A.D. Griffiths
Lab on a chip, 9, 2665-2672 (2009)
Link
Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis
Mazutis L., Fallah Araghi A., O.J. Miller, Baret J.-C., Frenz L, Janoshazi A., V. Taly, Miller B.J., Hutchison J.B., Link D.R., Griffiths A.D. and M. Ryckelynck
Analytical Chemistry, 81 (12), 4813-4821 (2009)
Link
Droplet and Emulsions: very high throughput in biology
Baret J.-C., V. Taly, M. Ryckelynck, C.A. Merten and A.D. Griffiths
Medecine Sciences, 25,627-632(2009)
Link
 |
Fluorescent Activated Droplet Sorting: Efficient Microfluidic cell sorting based on enzymatic activities Baret J.-C.,Miller O.J., Taly V., Ryckelynck M., El Harrak A., Frenz L., Rick C., Samuels M., Hutchison J.B., Agresti J.J., Link D.R., Weitz D.A., and Griffiths A.D. Lab on a chip, 9, 1850-1858 (2009) Link |
A competition-based assay for the screening of species-specific antibiotics
Granieri L., Miller O.J., A.D. Griffiths and C.A. Merten
Journal of antimicrobial chemotherapy, 64, (1) 62-68 (2009)
Link
Kinetic aspects of emulsion stabilization by surfactants: a microfluidic analysis
Baret J.-C., Kleinschmidt F., El Harrak A. and Griffiths A.D.
Langmuir, 25 (11), 6088-6093 (2009)
Link
 |
Reliable microfluidic on-chip incubation of droplets in delay-lines Frenz L., Blank K., Brouzes E., and Griffiths A.D. Lab on a Chip, 9, 1344 (2009) Link |
2008
Microfluidic production of droplet pairs
Frenz L., Blouwolff J., Griffiths A.D., and Baret J.-C.
Langmuir, 24(20), 12073 (2008) Link
Droplet-based microreactors for the synthesis of iron-oxide nanoparticles
Frenz L., El Harrak A., Pauly M., Begin-Colin S., Griffiths A.D., and Baret J.-C.
Angewandte Chemie Int. Ed. 47, 6817-6820 (2008) Link
Tropfenbasierte Mikroreaktoren fuer die Synthese von magnetischen Eisenoxid-Nanopartikeln
Frenz L., El Harrak A., Pauly M., Begin-Colin S., Griffiths A.D., and Baret J.-C.
Angewandte Chemie 120, 36, 6923-6926 (2008) Link
Drop-based microfluidic devices for encapsulation of single cells
Koster S., Angile F.E., Duan H., Agresti J.J., Wintner A., Schmitz C., Rowat A.C., Merten C.A., Pisignano D., Griffiths A.D., Weitz D.A.
Lab on a Chip, 8, 1110 (2008) Link
Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms
Clausell-Tormos J., Lieber D., Baret J.-C., El Harrak A., Miller O.J., Frenz L., Blouwolff J., Humphry K.J., Koster S., Duan H., Holtze C., Weitz D.A., Griffiths A.D. and Merten C.A.
Chemistry and Biology, 15, 427-437 (2008) Link
2007
Switching Liquid Morphologies on linear grooves
K. Khare, S. Herminghaus, J.-C. Baret, B. Law, M. Brinkmann and R. Seemann
Langmuir, vol.23 pp.12997-13006 (2007) Link
Miniaturizing chemistry and biology in microdroplets
B.T. Kelly, J.-C. Baret, V. Taly and A.D. Griffiths
Chem. Commun,1773-1788 (2007) Link
Droplets as micro-reactor for high-throughput biology
V. Taly, B.T. Kelly and A.D. Griffiths
Chem. Bio. Chem,8(3) 263-272 (2007) Link
2006
Directed evolution by in vitro compartmentalization
O.J. Miller, K.Bernath, J.J. Agresti, G. Amitai, B.T. Kelly, E. Mastrobattista, V.Taly, S. Magdassi, D.S. Tawfik and A.D. Griffiths
Nature Methods, vol(3)no.7, 561 (2006) Link
Amplification of complex gene librairies by emulsion PCR
R. Williams, S.G. Peisajovich, O.J. Miller, S. Magdassi, D.S. Tawfik and A.D. Griffiths
Nature Methods, vol(3) no.7, 545 (2006) Link
Analogues with Fluorescent Leaving groups for screening and selection of enzymes that efficiently hydrolyze organophosphorus nerve agents
L. Briseno-Roa, J. Hill, S. Notman, D. Sellers, A.P. Smith, C.T.Timperley, J. Wetherell, N.H. Williams, G.R. Williams, A.R. Fersht and A.D. Griffiths
J. Med. Chem., 47 246-255 (2006) Link
2005
Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization
J.J. Agresti, B.T. Kelly, A. J�schke and A.D. Griffiths
PNAS, 102 45 16710-16175 (2005) Link
High-throughput screening of enzymes librairies: In vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions
E. Mastrobattista, V. Taly, E. Chanudet, P. Treacy, B.T. Kelly and A.D. Griffiths
Chemistry and Biology, 12 1291-1300 (2005) Link
Determinants of cofactor binding to DNA methyltransferases: insights from a systematic series of structural variants of S-adenosylhomocysteine
H.M. Cohen, A.D. Griffiths, D.S. Tawfik and D. Loakes
Org. Biomol. Chem, 3, 152�161 (2005) Link
2004
Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalisation
H.M. Cohen, D.S. Tawfik & A.D. Griffiths
Protein Engineering Design and Selection, 17, 3-11, (2004) Link
In vitro compartmentalisation by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
K. Bernath, M. Hai, E. Mastrobattista, A.D. Griffiths, S. Magdassi & D. Tawfik
Analytical Biochemistry, 325: 151-157 (2004) Link
2003
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalisation
A.D. Griffiths and D.S. Tawfik.
EMBO J, 22: 24-35 (2003) Link
2002
Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase
H.M. Cohen, D.S. Tawfik and A.D. Griffiths
Nucleic Acids Res, 30, 3880-3885 (2002) Link
Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selelction using in vitro compartmentalisation.
Y.-F. Lee, D.S. Tawfik and A.D. Griffiths.
Nucleic Acids Res, 30, 4937-4944 (2002) Link
Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry
A. Sepp, D.S. Tawfik and A.D. Griffiths.
FEBS Letters, 532: 455-458 (2002) Link
1999
Interdomain interactions within the gene 3 protein of filamentous phage.
J. Chatellier, O. Hartley, A.D. Griffiths, A.R. Fersht, G. Winter and L. Riechmann.
FEBS Letters, 463: 371-374 (1999) Link
1998
Panning phage antibody libraries on cells: isolation of human Fab fragments against ovarian carcinoma using guided selection
M. Figini, L. Obici, D. Mezzanzanica, A. Griffiths, M.I. Colnaghi, G. Winter and S. Canevari
Cancer Res, 58, 991-996 (1998) Link
Small binding proteins selected from a combinatorial repertoire of knottins displayed on phage
G.P. Smith, S.U. Patel, J.D. Windass, J.M. Thornton, G. Winter and A.D. Griffiths
J. Mol. Biol, 277, 317-332 (1998) Link
Man-made cell-like compartments for molecular evolution
D.S. Tawfik and A.D. Griffiths
Nature Biotechnol, 16: 652-656 (1998) Link
1996
Characterization of human variable domain antibody fragments against the U1 RNA-associated A-protein, selected from a synthetic and a patient-derived combinatorial V-gene library
R.M.T. de Wildt, R. Finnern, W.H. Ouwehand, A.D. Griffiths, W.J. van Venrooij, R.M.A. Hoet.
Eur. J. Immunol, 26, 629-639 (1996) Link
Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin
J. Fan, A.D. Griffiths, A. Lockhart, R.A. Cross and L.A. Amos
J. Mol. Biol., 259: 325-330 (1996) Link
A strategy of exon shuffling for making large peptide repertoires displayed on filamentous bacteriophage
I. Fisch, R.E. Kontermann, R. Finnern, O. Hartley, A. S. Soler-Gonzalez, A.D. Griffiths and G. Winter.
Proc. Natl. Acad. Sci. USA, 93, 7761-7766 (1996) Link
1995
Phage antibodies against HGF/SF and other kringle containing proteins
M. Zaccolo, T.D. Prospero, A. Griffiths, G. Winter and E. Gherardi.
In Perspectives in Protein Engineering and Complementary Technologies. Ed. M.J. Geisow and R. Epton, pp. 170-171. Mayflower Worldwide, Birmingham (1995) Link
1994
In vitro assembly of repertoires of antibody chains on the surface of phage by renaturation M. Figini, J.D. Marks, G. Winter and A.D. Griffiths.
J. Mol. Biol., 239: 68-78 (1994) Link
Isolation of high affinity human antibodies directly from large synthetic repertoires
A.D. Griffiths, S.C. Williams, O. Hartley, I.M. Tomlinson, P. Waterhouse, W.L. Crosby, R.E. Kontermann, P.T. Jones, N.M. Low, T.J. Allison, T.D. Prospero, H.R. Hoogenboom, A. Nissim, J.P.L. Cox, J.L. Harrison, M. Zaccolo, E. Gherardi and G. Winter.
EMBO J., 13: 3245-3260 (1994) Link
1993
Human anti-self antibodies with high specificities from phage display libraries
A.D. Griffiths, M. Malmqvist, J.D. Marks, J.M. Bye, M.J. Embleton, J. McCafferty, M. Baier, K.P. Holliger, B.D. Gorick, N.C. Hughes-Jones, H.R. Hoogenboom and G. Winter.
EMBO J. 12: 725-734 (1993) Link
Combinatorial infection and in vivo recombination: a strategy for making large antibody repertoires
P. Waterhouse, A.D. Griffiths, K.S. Johnson and G. Winter
Nucl. Acids Res., 21: 2265-2266 (1993) Link
1992
By-passing immunization: building high affinity human antibodies by chain shuffling
J.D. Marks, A.D. Griffiths, M. Malmqvist, T.P. Clackson, J.M. Bye and G. Winter.
Bio/technology 10: 779-783 (1992) Link
1991
Making antibody fragments using phage display libraries.
T.Clackson, H.R. Hoogenboom, A.D. Griffiths and G. Winter.
Nature, 352:624-628 (1991) Link
[Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains H.R. Hoogenboom, A.D. Griffiths, K.S. Johnson, D.J. Chiswell, P. Hudson and Greg Winter.
Nucl. Acids Res., 19: 4133-4137 (1991) Link
Human antibodies from V-gene libraries displayed on phage
J.D. Marks, H.R. Hoogenboom, T.P. Bonnert, J. McCafferty, A.D. Griffiths and G. Winter.
J. Mol. Biol., 222: 581-597 (1991) Link
1990 - ...
Phage antibodies: filamentous phage displaying antibody variable domains.
J. McCafferty, A.D. Griffiths, G. Winter and D.J. Chiswell.
Nature, 348: 552-554 (1990). Link
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
E.S. Ward, D. G�ssow, A.D. Griffiths, P.T. Jones and G. Winter.
Nature, 341: 544-546 (1989) Link
Substitution of pre-mRNA with phosphorothioate linkages reveals a new splicing-related reaction
A.D. Griffiths, B.V.L. Potter and I.C. Eperon.
Journal of Biological Chemistry, 263: 12295-12304 (1988) Link
Effects of RNA secondary structure on alternative splicing of pre-mRNA: is folding limited to a region behind the transcribing RNA polymerase?
L.P. Eperon, I.R. Graham, A.D. Griffiths and I.C. Eperon. (1988).
Cell, 54: 393-401. Link
Stereospecificity of nucleases towards phosphorothioate - substituted RNA: stereochemistry of transcription by T7 RNA polymerase
A.D. Griffiths, B.V.L. Potter and I.C. Eperon.
Nucleic Acids Res. 15: 4145-4162 (1987) Link